Avantor® ACE® Knowledge notes

This Knowledge Note explains how to calculate and experimentally determine column volume and porosity.

Extra column volume and dispersion can seriously affect column performance. Here we explain why, how to experimentally measure and approaches to help reduce their impact.

The free to download ACE LC Translator can be used to simplify translating gradient methods between different format columns.

Determining the dwell volume of an LC system is very useful as it is the basis of gradient elution method transfer.

Have you ever been confused as to the best solution to a certain separation problem? We explain the effect of these parameters on performance.

One of the key variables in you method development is stationary phase selectivity. You can learn more about this important tool.

If you are looking for a clear introduction to the practicalities of HILIC, this is a comprehensive guide for those unfamiliar with this mode of HPLC.

How you can create the correct environment for the analysis of monosaccarides and reducing disaccharides.

Your choice of sample diluent in HILIC can have a profound effect on the chromatography. This note explains why.

It is essential to fully equilibrate your HILIC columns prior to analysis to ensure a stable adsorbed water layer

Learn how UHPLC can be used to dramatically increase sample throughput and produce ultra-high resolution separations of complex samples.

This Knowledge Note introduces the key concepts of gradient chromatography and explains how to determine appropriate gradient conditions for a separation.