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Dihydrorhodamine 123 ≥95% (by HPLC), Sigma-Aldrich®

Fornitore: SIGMA-ALDRICH MICROSCOPY

Descrizione: Dihydrorhodamine 123 has been used for the detection of free intracellular radicals or ROS (reactive oxygen species).

Dihydrorhodamine 123 ≥95% (by HPLC), ultrapuro

Codice catalogo: (ENZOENZ52302)

Fornitore: ENZO LIFE SCIENCES AG

Codice articolo fornitore: ENZ52302

Codice articolo locale: ENZOENZ52302

Descrizione: Mitochondria dye

UOM: 1 * 10 mg

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Reactive oxygen species indicators dyes

Fornitore: Biotium

Descrizione: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

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Neutrophil/Monocyte Respiratory Burst Assay Kit

Codice catalogo: (CAYM601130-1)

Fornitore: Cayman Chemical

Codice articolo fornitore: 601130-1

Codice articolo locale: CAYM601130-1

Descrizione: Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

UOM: 1 * 1 KIT

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