Hai cercato: Acido+4-indolil-3-butirrico

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X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galattopiranoside)

Fornitore: G-Biosciences

Descrizione: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

n-Butirronitrile ≥99.0% (by GC)

Fornitore: TCI

Descrizione: n-Butirronitrile ≥99.0% (by GC)

n-Butirronitrile 99

Fornitore: Thermo Fisher Scientific

Descrizione: n-Butirronitrile 99

MSDS Certificati

n-Butirronitrile 99%

Fornitore: Thermo Fisher Scientific

Descrizione: n-Butirronitrile 99%

MSDS

X-GlcA

Codice catalogo: (GENORC-214)

Fornitore: G-Biosciences

Codice articolo fornitore: RC-214

Codice articolo locale: GENORC-214

Descrizione: X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid) is a substrate for ß-glucuronidase (GUS), which is encoded by <italic>gusA</italic>, a widely used reporter gene. The glucuronidase cleaves X-Gluc to produce colourless glucuronic acid and an intense blue precipitate of chloro-bromoindigo. X-Gluc is used for the histochemical detection of GUS expression in plant cells and tissues, detection of infections caused by <italic>E.coli</italic> and detection of bacterial contamination in food and water samples.

UOM: 1 * 100 mg

Promo

Non disponibile confezione

X-Gal (5-Bromo-4-cloro-3-indolil-ß-D-galattopiranoside) ≥98%, white powder per biologia molecolare

Fornitore: MP Biomedicals

Descrizione: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.