Hai cercato: Materiali didattici di biologia

Codice catalogo: (BE-404)

Fornitore: G-Biosciences

Codice articolo fornitore: BE-404

Codice articolo locale: GENOBE-404

Descrizione: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (BE-401)

Fornitore: G-Biosciences

Codice articolo fornitore: BE-401

Codice articolo locale: GENOBE-401

Descrizione: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (BE-502)

Fornitore: G-Biosciences

Codice articolo fornitore: BE-502

Codice articolo locale: GENOBE-502

Descrizione: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (EDVO124)

Fornitore: EDVOTEK

Codice articolo fornitore: 124

Codice articolo locale: EDVO124

Descrizione: This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (EDVO221)

Fornitore: EDVOTEK

Codice articolo fornitore: 221

Codice articolo locale: EDVO221

Descrizione: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (EDVO314)

Fornitore: EDVOTEK

Codice articolo fornitore: 314

Codice articolo locale: EDVO314

Descrizione: Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (EDVO333)

Fornitore: EDVOTEK

Codice articolo fornitore: 333

Codice articolo locale: EDVO333

Descrizione: Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

UOM: 1 * 1 KIT

Promo

Fornitore: EDVOTEK

Descrizione: Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.

Codice catalogo: (EDVO953)

Fornitore: EDVOTEK

Codice articolo fornitore: 953

Codice articolo locale: EDVO953

Descrizione: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (EDVO274)

Fornitore: EDVOTEK

Codice articolo fornitore: 274

Codice articolo locale: EDVO274

Descrizione: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

UOM: 1 * 1 pezzi

MSDS Promo

Codice catalogo: (EDVO153)

Fornitore: EDVOTEK

Codice articolo fornitore: 153

Codice articolo locale: EDVO153

Descrizione: Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.

UOM: 1 * 1 KIT

MSDS Promo

Codice catalogo: (EDVO207)

Fornitore: EDVOTEK

Codice articolo fornitore: 207

Codice articolo locale: EDVO207

Descrizione: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

UOM: 1 * 1 pezzi

Promo

Fornitore: EDVOTEK

Descrizione: Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

Codice catalogo: (EDVO908)

Fornitore: EDVOTEK

Codice articolo fornitore: 908

Codice articolo locale: EDVO908

Descrizione: Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (EDVO300)

Fornitore: EDVOTEK

Codice articolo fornitore: 300

Codice articolo locale: EDVO300

Descrizione: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (EDVO223/AP08)

Fornitore: EDVOTEK

Codice articolo fornitore: 223/AP08

Codice articolo locale: EDVO223/AP08

Descrizione: In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish <i>Aequorea victoria</i>. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

UOM: 1 * 1 pezzi

Promo