Hai cercato: Estrazione delle proteine

Fornitore: Thermo Fisher Scientific

Descrizione: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Codice catalogo: (PIER20230)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 20230

Codice articolo locale: PIER20230

Descrizione: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.

UOM: 1 * 2 mL

Promo

Codice catalogo: (PIER28340)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 28340

Codice articolo locale: PIER28340

Descrizione: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER89801)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 89801

Codice articolo locale: PIER89801

Descrizione: The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (786-792)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-792

Codice articolo locale: GENO786-792

Descrizione: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (786-232)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-232

Codice articolo locale: GENO786-232

Descrizione: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Certificati

Codice catalogo: (PIER89874)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 89874

Codice articolo locale: PIER89874

Descrizione: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER89839)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 89839

Codice articolo locale: PIER89839

Descrizione: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.

UOM: 1 * 1 KIT

Promo

Fornitore: Merck Millipore (Novagen)

Descrizione: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Fornitore: Merck Millipore (Novagen)

Descrizione: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Codice catalogo: (324715-5)

Fornitore: Merck Millipore (Calbiochem‎)

Codice articolo fornitore: 324715-5

Codice articolo locale: CALB324715-5

Descrizione: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.

UOM: 1 * 5 µG

Promo

Codice catalogo: (786-125)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-125

Codice articolo locale: GENO786-125

Descrizione: The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.

UOM: 1 * 50 mL

Promo

Codice catalogo: (786-316)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-316

Codice articolo locale: GENO786-316

Descrizione: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (324713-50)

Fornitore: Merck Millipore (Calbiochem‎)

Codice articolo fornitore: 324713-50

Codice articolo locale: CALB324713-50

Descrizione: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

UOM: 1 * 50 µG

Promo

Fornitore: Merck Millipore (Novagen)

Descrizione: Il reagente PopCulture® è un concentrato a base di detergente che può essere aggiunto direttamente alle colture di E. coli per estrarre efficacemente proteine senza la necessità della raccolta di cellule. Le proteine ricombinanti possono sottoposte a screening direttamente nell'estratto grezzo oppure purificate mediante l'aggiunta di una matrice di affinità, il lavaggio del complesso matrice-proteine target per rimuovere il terreno di coltura esaurito e i contaminanti cellulari, nonché l'eluizione della proteina purificata dalla matrice. La procedura di purificazione delle proteine PopCulture è ideale per l'elaborazione robotica ad alta resa di campioni per la ricerca proteomica o tutte le applicazioni che trarrebbero vantaggio dal miglioramento di velocità e praticità.