Hai cercato: Reagenti per estrazione di proteine

Fornitore: ENZO LIFE SCIENCES AG

Descrizione: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Codice catalogo: (786-244)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-244

Codice articolo locale: GENO786-244

Descrizione: SilverOUT removes interfering silver ions from gel pieces.

UOM: 1 * 100 Tests

Promo

Codice catalogo: (786-793)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-793

Codice articolo locale: GENO786-793

Descrizione: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

UOM: 1 * 5 mL

Promo

Codice catalogo: (786-598)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-598

Codice articolo locale: GENO786-598

Descrizione: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

UOM: 1 * 5 mL

Promo

Codice catalogo: (786-681)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-681

Codice articolo locale: GENO786-681

Descrizione: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (DGA01)

Fornitore: G-Biosciences

Codice articolo fornitore: DGA01

Codice articolo locale: GENODGA01

Descrizione: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

UOM: 1 * 500 pezzi

Promo

Codice catalogo: (PIER90054)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 90054

Codice articolo locale: PIER90054

Descrizione: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

UOM: 1 * 50 µG

MSDS Promo

Codice catalogo: (PIER90056)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 90056

Codice articolo locale: PIER90056

Descrizione: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

UOM: 1 * 100 µG

Promo

Codice catalogo: (786-599)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-599

Codice articolo locale: GENO786-599

Descrizione: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

UOM: 1 * 5 mL

Promo

Codice catalogo: (PIER90053)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 90053

Codice articolo locale: PIER90053

Descrizione: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

UOM: 1 * 2 µG

MSDS Promo

Codice catalogo: (786-241)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-241

Codice articolo locale: GENO786-241

Descrizione: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

UOM: 1 * 1 KIT

Promo

Fornitore: G-Biosciences

Descrizione: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Fornitore: G-Biosciences

Descrizione: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Certificati

Codice catalogo: (ENZOBMLKI1050300)

Fornitore: ENZO LIFE SCIENCES AG

Codice articolo fornitore: BMLKI1050300

Codice articolo locale: ENZOBMLKI1050300

Descrizione: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

UOM: 1 * 300 µl

New Product Promo

Codice catalogo: (PIER90067)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 90067

Codice articolo locale: PIER90067

Descrizione: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

UOM: 1 * 1 KIT

Promo

Fornitore: G-Biosciences

Descrizione: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.