DreamTaq™ Green DNA Polymerase
Fornitore: Thermo Fisher Scientific
DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.
- Save time – go directly from PCR to gel electrophoresis
- High yields of PCR products with minimal optimisation
- Higher sensitivity compared to conventional Taq DNA polymerase
- Amplification of long targets (up to 6 kb from genomic DNA, up to 20 kb from viral DNA)
- Robust amplification of difficult templates
Informazioni per l'ordine: Supplied with 10X DreamTaq™ Green Buffer, which includes 20 mM MgCl₂.
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