Hai cercato: Thermo Fisher Scientific

Fornitore: Thermo Fisher Scientific

Descrizione: Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.

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Fornitore: Thermo Fisher Scientific

Descrizione: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

MSDS

Codice catalogo: (R0631)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: R0631

Codice articolo locale: FERMR0631

Descrizione: Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Codice catalogo: (FD1704)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: FD1704

Codice articolo locale: FERMFD1704

Descrizione: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (R0611)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: R0611

Codice articolo locale: FERMR0611

Descrizione: This gel loading dye contains two different dyes bromophenol blue and xylene cyanol FF for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.

UOM: 1 * 1 KIT

Certificati Promo

Fornitore: Thermo Fisher Scientific

Descrizione: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Certificati

Fornitore: Thermo Fisher Scientific

Descrizione: Human ribonuclease inhibitor (from placenta)

Fornitore: Thermo Fisher Scientific

Descrizione: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Fornitore: Thermo Fisher Scientific

Descrizione: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Codice catalogo: (ER1751)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: ER1751

Codice articolo locale: FERMER1751

Descrizione: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Fornitore: Thermo Fisher Scientific

Descrizione: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

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Codice catalogo: (K1441)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: K1441

Codice articolo locale: FERMK1441

Descrizione: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Certificati

Fornitore: Thermo Fisher Scientific

Descrizione: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

MSDS