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Affinity chromatography resin, chelating nickel

Fornitore: G Biosciences

786-281 786-407 786-408 786-392 786-393 786-394
786-281EA 206 EUR
786-281 786-407 786-408 786-392 786-393 786-394
Affinity chromatography resin, chelating nickel
Purificazione proteine Resine per la purificazione delle proteine

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.


Nickel chelating resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20 to 40 μmoles Ni²⁺/ml resin. The protein binding capacity is >50 mg protein per ml resin. We have demonstrated binding of >100 mg of a 50 kDa 6X His tagged proteins to a ml of resin. The spin columns are supplied with a resin bed volume of 0,2, 1 and 3 ml with total column volumes of 1, 8 and 22 ml respectively. Columns can be used as a spin format of gravity flow columns.

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