Hai cercato: Tonbo Biosciences

Codice catalogo: (TNB-6600-KIT)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: TNB-6600-KIT

Codice articolo locale: TNBOTNB-6600-KIT

Descrizione: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

UOM: 1 * 50 Tests

Promo

Fornitore: Tonbo Biosciences

Descrizione: The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

Codice catalogo: (35-8023-M001)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 35-8023-M001

Codice articolo locale: TNBO35-8023-M001

Descrizione: Goat anti-Human IgA (alpha chain) - Affinity Pure, FITC Conjugate, min x w/bovine, mouse, and rabbit serum proteins

UOM: 1 * 1 mg

Promo

Codice catalogo: (30-8065-M002)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 30-8065-M002

Codice articolo locale: TNBO30-8065-M002

Descrizione: Goat anti-Mouse IgM (mu chain) - Affinity Pure, Biotin Conjugate

UOM: 1 * 2 mg

Promo

Codice catalogo: (30-8064-M002)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 30-8064-M002

Codice articolo locale: TNBO30-8064-M002

Descrizione: Goat anti-Mouse IgM (H&L) - Affinity Pure, Biotin Conjugate

UOM: 1 * 2 mg

Promo

Codice catalogo: (70-8078-M002)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 70-8078-M002

Codice articolo locale: TNBO70-8078-M002

Descrizione: Sheep anti-Rabbit IgG (H&L) - Affinity Pure

UOM: 1 * 2 mg

Promo

Codice catalogo: (72-8074-U500)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 72-8074-U500

Codice articolo locale: TNBO72-8074-U500

Descrizione: Goat anti-Rabbit IgG (H&L), F(ab')2 Fragment - Affinity Pure, HRP Conjugate, min x w/ bovine, human, or mouse IgG or serum proteins

UOM: 1 * 0,5 mg

Promo

Fornitore: Tonbo Biosciences

Descrizione: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.

Fornitore: Tonbo Biosciences

Descrizione: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.

Codice catalogo: (TNB-4700-L100)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: TNB-4700-L100

Codice articolo locale: TNBOTNB-4700-L100

Descrizione: Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

UOM: 1 * 100 mL

Promo

Fornitore: Tonbo Biosciences

Descrizione: The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others. The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Fornitore: Tonbo Biosciences

Descrizione: The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

Fornitore: Tonbo Biosciences

Descrizione: The UCHT2 antibody is specific for human CD5, a 67 kD transmembrane glycoprotein, that is expressed on most thymocytes, mature T cells, and a subset of B cells. CD5 is involved in modulating antigen receptor signaling in both T and B cells and binds to CD72, expressed on B cells.

Codice catalogo: (72-8078-M001)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 72-8078-M001

Codice articolo locale: TNBO72-8078-M001

Descrizione: Sheep anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate

UOM: 1 * 1 mg

Promo

Codice catalogo: (72-8068-M001)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 72-8068-M001

Codice articolo locale: TNBO72-8068-M001

Descrizione: Goat anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/ bovine, goat, human, mouse or rat IgG or serum

UOM: 1 * 1 mg

Promo

Codice catalogo: (50-8036-U100)

Fornitore: Tonbo Biosciences

Codice articolo fornitore: 50-8036-U100

Codice articolo locale: TNBO50-8036-U100

Descrizione: Goat anti-Rabbit IgG, secondary antibody, conjugated toSureLight® R-Phycoerythrin

UOM: 1 * 100 µG

Promo