Hai cercato: Merck Millipore (Novagen)

Fornitore: Merck Millipore (Novagen)

Descrizione: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Fornitore: Merck Millipore (Novagen)

Descrizione: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Codice catalogo: (70856-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 70856-3

Codice articolo locale: NOVG70856-3

Descrizione: Convenient solution for selective degradation of RNA.

UOM: 1 * 1 mL

Promo

Fornitore: Merck Millipore (Novagen)

Descrizione: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Codice catalogo: (69750-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 69750-3

Codice articolo locale: NOVG69750-3

Descrizione: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

Promo

Codice catalogo: (69704-4)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 69704-4

Codice articolo locale: NOVG69704-4

Descrizione: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

Promo

Fornitore: Merck Millipore (Novagen)

Descrizione: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Codice catalogo: (70527-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 70527-3

Codice articolo locale: NOVG70527-3

Descrizione: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems.

UOM: 1 * 15 mL

Promo

Codice catalogo: (69755-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 69755-3

Codice articolo locale: NOVG69755-3

Descrizione: The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (71281-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 71281-3

Codice articolo locale: NOVG71281-3

Descrizione: ProteoJuice™ protein transfection reagent is an effective reagent for the transfection of intact functional protein and peptides into mammalian cells with minimal toxicity and broad cell specificity.

UOM: 1 * 1 pezzi

Promo

Codice catalogo: (71285-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 71285-3

Codice articolo locale: NOVG71285-3

Descrizione: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 KIT

Promo

Fornitore: Merck Millipore (Novagen)

Descrizione: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Fornitore: Merck Millipore (Novagen)

Descrizione: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Fornitore: Merck Millipore (Novagen)

Descrizione: Il reagente per estrazione proteica BugBuster®  è formulato espressamente per una lisi delicata della parete cellulare dell'E. coli e per la conseguente liberazione delle proteine solubili. Questo reagente rappresenta un'alternativa semplice, rapida ed economica ai metodi meccanici (frammentazione a pressione 'French Press' o sonicazione) per il rilascio delle proteine bersaglio prima della purificazione o di altre applicazioni. Nella sua formulazione, il reagente utilizza una miscela di detergenti in grado di perforare la parete cellulare senza denaturare le proteine solubili.

Codice catalogo: (71546-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 71546-3

Codice articolo locale: NOVG71546-3

Descrizione: The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (69022-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 69022-3

Codice articolo locale: NOVG69022-3

Descrizione: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

UOM: 1 * 1 KIT

Promo