Hai cercato: Reagenti per Western Blotting

Codice catalogo: (PIER34070)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34070

Codice articolo locale: PIER34070

Descrizione: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.

UOM: 1 * 100 mL

Promo

Codice catalogo: (PIER34000)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34000

Codice articolo locale: PIER34000

Descrizione: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (786-423)

Fornitore: G-Biosciences

Codice articolo fornitore: 786-423

Codice articolo locale: GENO786-423

Descrizione: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.

UOM: 1 * 1 L

Promo

Codice catalogo: (PIER15162)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 15162

Codice articolo locale: PIER15162

Descrizione: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER37574)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 37574

Codice articolo locale: PIER37574

Descrizione: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

UOM: 1 * 250 mL

Promo

Fornitore: AAT BIOQUEST

Descrizione: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

New Product

Codice catalogo: (PIER34012)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34012

Codice articolo locale: PIER34012

Descrizione: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

UOM: 1 * 250 mL

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

MSDS

Fornitore: Thermo Fisher Scientific

Descrizione: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Codice catalogo: (PIER34002)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34002

Codice articolo locale: PIER34002

Descrizione: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER17075)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 17075

Codice articolo locale: PIER17075

Descrizione: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER31490)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 31490

Codice articolo locale: PIER31490

Descrizione: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

UOM: 1 * 10 mg

Promo

Codice catalogo: (PIER15165)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 15165

Codice articolo locale: PIER15165

Descrizione: HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

UOM: 1 * 2 mg

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.

Fornitore: Thermo Fisher Scientific

Descrizione: SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

Fornitore: Thermo Fisher Scientific

Descrizione: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.