Acriflavine fluorescent labeling reagent, Sigma-Aldrich®
Fornitore: SIGMA-ALDRICH MICROSCOPY
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Attenzione
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Synonyms:
3,6-Diamino-10-methylacridinium chloride-3,6-acridinediamine mixt, Acriflavinium chloride 3,6-Acridinediamine mix
M-Clarity™ quality level = MQ200
Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.
- Acriflavine is used to fluorescently label high molecular weight RNA molecules
- Excitation and emission wavelengths in various solvents:
- Methanol: λex = 424 nm; λem = 518 nm
- Ethanol: λex = 426 nm; λem = 524 nm
- Propanol: λex = 430 nm; λem = 512 nm
- Butanol: λex = 430 nm; λem = 526 nm
- Formamide: λex = 434 nm; λem = 524 nm
- Glycerol: λex = 432 nm; λem = 540 nm
- Water: λex = 416 nm; λem = 514 nm
Insoluble in ether, chloroform, and fixed oils. Utilised in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor.
Acriflavine has been used in the agglutination test to distinguish between smooth and rough colony formation of Brucella melitensis.
It has been used as an AgO₂ (argonaute 2) inhibitor.
It has been used as an inhibitor of HIF-ARNT (hypoxia-inducible factor - aryl hydrocarbon receptor nuclear translocator) complex formation.
It has been used to study the bacteriocin production by Carnobacterium piscicola.
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Formula:
C₂₇H₂₅ClN₆ Peso molecolare: 468,9806 g/mol Punto di fusione: 179,..181 °C Temperatura di stoccaggio: Room Temperature |
MDL Number:
MFCD00064307 CAS Number: 8048-52-0 |
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Risultati del test di specificazione
| Appearance (Form) | Powder |
| Proton NMR spectrum | Conforms to Structure |
| EmM, 462 nm (+/- 3 nm), C = 0.004 g/L in Methanol | ≥48000 |
| Wavelength | 259 - 265 nm |
| EmM, 262 nm (+/- 3 nm), C = 0.004 g/L in Methanol | ≥50000 |
| Solubility (Colour) | Orange to Brown |
| Solubility (Turbidity), 50 mg/mL in H₂O | Clear |
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