Fura 2-AM ≥95% (by HPLC)
Fornitore: SIGMA-ALDRICH MICROSCOPY
Synonyms:
FURA-2 pentakis(acetoxymethyl) ester, 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester, FURA-2 acetoxymethyl ester, Fura-2 AM Ester
M-Clarity™ quality level = MQ100
Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505 nm, and the excitation peak ranges between 340 to 380 nm and is the preferred calcium indicator.
- Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells
The major benefits of using the ester form of Fura 2 as Fura 2-AM are: It is cell-permeable. It can be loaded into cells easily. Another advantage of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : The dye concentration. Illumination intensity, and Optical path length.
Formula:
C₄₄H₄₇N₃O₂₄ Peso molecolare: 1001,857 g/mol Temperatura di stoccaggio: Congelamento |
MDL Number:
MFCD00036976 CAS Number: 108964-32-5 |
Risultati del test di specificazione
Appearance (Colour) | Yellow to Yellow-Green |
Appearance (Form) | Powder |
Solubility (Colour) | Yellow to Yellow-Green |
Solubility (Turbidity) 10 mg/ml, acetone |
Clear |
Purity (HPLC) | ≥95% |
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