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VWR®, Fast HiFi DNA Polymerase, 2 U/µl

Fornitore: VWR Chemicals

733-2880EA 133 EUR
733-2880 733-2881 733-2882 733-2883
VWR®, Fast HiFi DNA Polymerase, 2 U/µl
Reagenti per acidi nucleici Enzimi e kit per PCR end-point

Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.


  • High fidelity: >60X Taq Fidelity
  • 3’→5’ proof reading exonuclease activity
  • Processes up to 18 kb from complex templates (e.g. human gDNA)
  • Good coverage on DNA templates with low to high GC content
  • Fast extension rate: 10 s/kb


Fast HiFi DNA Polymerase 2 U/µl exhibits both 5'→3' DNA polymerase activity and 3'→5' proof reading exonuclease activity enabling this polymerase to correct base pair mismatches. It is supported with 5X Fast HiFi Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 to 2 M Betaine enhancer solution is recommended.

For more convenient handling Fast HiFi DNA Polymerase 2 U/µl is also available as a 2X master mix.

Sample types: gDNA, plasmid DNA, bacterial DNA, λDNA and other DNA templates


Attenzione: For Research Use Only. Not for use in diagnostic procedures.

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Specifiche

Descrizione Fast HiFi DNA Polymerase 2 U/µl 100 units Fast HiFi DNA Polymerase 2 U/µl 500 units Fast HiFi DNA Polymerase 2 U/µl 1000 units Fast HiFi DNA Polymerase 2 U/µl 2500 units
Formato Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Environmentally Preferable
Caratteristiche feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143 feature-ls-33964141|feature-ls-33964143
Avviamento a caldo No No No No
Concentrazione 2 U/µl 2 U/µl 2 U/µl 2 U/µl
Unità 100 500 1000 2500
Taglia 1×0,05 ml + 2×1,5 ml 1×0,25 ml + 3×1,5 ml 2×0,25 ml + 6×1,5 ml 5×0,25 ml + 14×1,5 ml
Composizione del tampone 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂ 5X buffer with 7,5 mM MgCl₂
Alta fedeltà Yes Yes Yes Yes
Dimensioni amplicone 18 kb for gDNA 18 kb for gDNA 18 kb for gDNA 18 kb for gDNA
Processivity Fast Fast Fast Fast
Attività di esonucleasi 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’ 5’→3’ 3’→5’
Product overhang No No No No
Condizioni di conservazione –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months) –20 °C (long-term), +4 °C (6 months)
Applicazione Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification Cloning (high fidelity),Standard amplification

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