Betaine enhancer solution 5 M (5X) for PCR
Fornitore: VWR Chemicals
Synonyms:
TMG
L'intensificatore alla betaina è particolarmente efficace se utilizzato in caso di templati ad elevato contenuto GC con alto grado di strutture secondarie. Ha un-effetto di decremento sulla temperatura di melting dei primer di fusione del primer.
- Enhances amplification on GC-rich and difficult DNA targets
- Lowers the melting temperature
The performance of Betaine Enhancer Solution 5 M is superior compared to standard enhancers such as formamide, DMSO, TMAC, BSA and non-ionic detergents. Betaine Enhancer Solution is an excellent enhancer especially when used with GC-rich regions or templates with a high degree of secondary structures.
Betaine has a decreasing effect on the melting temperature of DNA and primers. Therefore, denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5 °C. The optimal annealing temperature should be determined individually for each reaction.
Attenzione: For Research Use Only. Not for use in diagnostic procedures.
Formula:
C₅H₁₁NO₂ Peso molecolare: 117,15 g/mol Temperatura di stoccaggio: Congelamento |
MDL Number:
MFCD00012123 CAS Number: 107-43-7 |
Risultati del test di specificazione
Functional test - 1x Betaine Enchancer Solution is tested for amplification of 613bp fragment from human genomic DNA using Taq DNA polymerase at standard PCR Conditions | Passes test |
Volume check- fill volume >= 0.1ml per tube | Passes test |
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