Serine hydrolase probes, ActivX™ FP
Fornitore: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
- Specifically labels the reactive site of active serine hydrolases
- Compatible tags available for capture, detection and Staudinger conjugation
- Use for in vitro or intracellular enzyme labeling
Azido-FP probes are used in combination with phosphine- or alkyne-derivatised tags for detection or enrichment.
Desthiobiotin-FP probes are used for enrichment and detection of active site-labeled proteins by Western blot or mass spectrometry.
TAMRA-FP probes are used to label and detect serine hydrolase activity in samples using fluorescent gel imaging, capillary electrophoresis or mass spectrometry.
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