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Trypan blue 0,4% in soluzione acquosa, Sigma-Aldrich®

Fornitore: SIGMA-ALDRICH MICROSCOPY
Pericolo

Synonyms: Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulphonic acid tetrasodium salt, CI 23850, 3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulphonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium salt, Benzamine blue, Trypan blue sodium salt, Direct Blue 14, Diphenyl Blue, AzidinBlue, Sodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulphonate)

M-Clarity™ quality level = MQ200

93595-250ML 93595-50ML
93595-250MLEA 81.7 EUR
93595-250ML 93595-50ML
Trypan blue 0,4% in soluzione acquosa, Sigma-Aldrich®
Trypan blue

Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.


  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes


Physical form: 0,4% in 0,81% sodium chloride; 0,06% potassium phosphate, dibasic.

Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Peso molecolare: 960,82 g/mol
Densità: 1,007 g/ml at 20 °C
Temperatura di stoccaggio: Room Temperature
MDL Number: MFCD00003969
CAS Number: 72-57-1
EINECS: 200-786-7
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Risultati del test di specificazione

Appearance (colour) Blue to very dark blue
Appearance (form) Liquid
Thin layer chromatogr. Corresponds to standard
Density d20/4 0.997 - 1.017
Wavelength (1) (UV) 600 - 610 nm
Extinction (1%/1 cm) (1) (UV)
Solvent (UV) Methanol
Expiry period 30 months

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