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Hai cercato: Saggi proteici

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-733-1995

Protein quantitation assay kits, CB™ Protein assay

Fornitore: G-Biosciences
Descrizione: CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Product Image-786-12XT

Protein quantitation assay kits, CB-X™ Protein assay

Fornitore: G-Biosciences
Descrizione: CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

Image Unavailable-USBIO8061-100

Osteoclast inhibitory lectin MAB MO XMO

Codice catalogo: (USBIO8061-100)
Fornitore: US Biological
Codice articolo fornitore: O8061-100
Codice articolo locale: USBIO8061-100
Descrizione: Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
UOM: 1 * 100 µG
Product Image-PIER23234

Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Fornitore: Thermo Fisher Scientific
Descrizione: Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

Product Image-PIER16175

Firefly luciferase flash assay kit, Pierce™

Fornitore: Thermo Fisher Scientific
Descrizione: The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Product Image-PIER48895

Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Codice catalogo: (PIER48895)
Fornitore: Thermo Fisher Scientific
Codice articolo fornitore: 48895
Codice articolo locale: PIER48895
Descrizione: Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
UOM: 1 * 50 mg
Product Image-PIER23215

Compat-Able™ Protein assay preparation reagent set

Codice catalogo: (PIER23215)
Fornitore: Thermo Fisher Scientific
Codice articolo fornitore: 23215
Codice articolo locale: PIER23215
Descrizione: The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 500 mL
Image Unavailable-PIER23239

Coomassie Plus™ protein assay kit, Compat-Able™

Codice catalogo: (PIER23239)
Fornitore: Thermo Fisher Scientific
Codice articolo fornitore: 23239
Codice articolo locale: PIER23239
Descrizione: The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 1 KIT
Image Unavailable-71840-3

Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Codice catalogo: (71840-3)
Fornitore: Merck Millipore (Novagen)
Codice articolo fornitore: 71840-3
Codice articolo locale: NOVG71840-3
Descrizione: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
UOM: 1 * 125 µl
Product Image-PIER23212

Pierce™ BCA™ Protein assay kits and reagents

Fornitore: Thermo Fisher Scientific
Descrizione: Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Product Image-PIER23240

Modified lowry protein assay kit, Pierce™

Codice catalogo: (PIER23240)
Fornitore: Thermo Fisher Scientific
Codice articolo fornitore: 23240
Codice articolo locale: PIER23240
Descrizione: The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.
UOM: 1 * 1 KIT

MSDS Certificati


Image Unavailable-AATB11109

Portelite™ Fluorimetric Protein Quantitation Kit

Codice catalogo: (AATB11109)
Fornitore: AAT Bioquest
Codice articolo fornitore: 11109
Codice articolo locale: AATB11109
Descrizione: Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.
UOM: 1 * 100 Tests

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Image Unavailable-AATB5539

Portelite™ Rapid Fluorimetric Biotin Quantitation Kit

Codice catalogo: (AATB5539)
Fornitore: AAT Bioquest
Codice articolo fornitore: 5539
Codice articolo locale: AATB5539
Descrizione: Optimised to use with CytoCite™ and Qubit™ fluorometers. Portelite™ rapid fluorimetric biotin quantitation kit is specifically optimised to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ fluorometer.
UOM: 1 * 50 Tests

New Product


Image Unavailable-PIER23290

Quantitative fluorometric peptide assay, Pierce™

Fornitore: Thermo Fisher Scientific
Descrizione: Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

Product Image-PIER23200

Coomassie (Bradford) protein assay kit, Pierce™

Codice catalogo: (PIER23200)
Fornitore: Thermo Fisher Scientific
Codice articolo fornitore: 23200
Codice articolo locale: PIER23200
Descrizione: The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
UOM: 1 * 1 KIT

MSDS Certificati


Product Image-PIER23250

Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Fornitore: Thermo Fisher Scientific
Descrizione: This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

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