Hai cercato: Reagenti RT-PCR e cDNA

Codice catalogo: (1B1326-50RXN)

Fornitore: VWR Chemicals

Codice articolo locale: VWRC1B1326-50RXN

Descrizione: Ready One-Step RT-PCR Kit offers a single step procedure for performing RT-PCR reactions. Subsequent loading and visualisation of RT-PCR products are streamlined since the loading buffer and visualisation dye are included. The user supplies the primers and the DNase-treated template RNA.

UOM: 1 * 1 KIT

Promo

Fornitore: Quantabio

Descrizione: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Fornitore: Quantabio

Descrizione: I kit qScript™ cDNA Synthesis contengono qScript™ Reverse Transcriptase, una miscela di MMLV ingegnerizzata e proteina per l'inibizione della ribonucleasi, ottimizzata per una sintesi di cDNA sensibile e affidabile. I reagenti per la sintesi di cDNA sono forniti in diversi formati supermix e in kit per soddisfare le esigenze specifiche delle singole applicazioni.

MSDS Certificati

Fornitore: Quantabio

Descrizione: La One-Step Kit RT-PCR qScript ™ XLT è un sistema conveniente e altamente sensibile per l'amplificazione di RNA modelli fino a 2 kb. sintesi del cDNA e l'amplificazione PCR vengono effettuate nello stesso tubo senza aprire tra procedure. Questo sistema è stato ottimizzato per garantire la massima RT-PCR efficienza, sensibilità e specificità.

Certificati

Fornitore: AGILENT

Descrizione: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Fornitore: ENZO LIFE SCIENCES AG

Descrizione: AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

New Product

Codice catalogo: (733-2013)

Fornitore: Quantabio

Codice articolo fornitore: 95107-025

Codice articolo locale: QUNT95107-025

Descrizione: The qScript™ microRNA cDNA synthesis kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs. miRNAs are not polyadenylated in nature. With the qScript™ microRNA cDNA synthesis kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript™ reverse transcriptase and other necessary reagents for cDNA synthesis are subsequently added to convert the poly(A) tailed microRNAs into cDNA using an oligo-dT adapter primer. The adapter primer has a unique sequence at its 5’ end which allows amplification of cDNAs in Real-Time qRT-PCR reactions.

UOM: 1 * 25 Assays

Promo

Fornitore: Quantabio

Descrizione: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

MSDS

Fornitore: ENZO LIFE SCIENCES AG

Descrizione: AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

New Product

Fornitore: Thermo Fisher Scientific

Descrizione: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

MSDS

Fornitore: Thermo Fisher Scientific

Descrizione: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Fornitore: Thermo Fisher Scientific

Descrizione: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Fornitore: Thermo Fisher Scientific

Descrizione: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Codice catalogo: (K1612)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: K1612

Codice articolo locale: FERMK1612

Descrizione: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Fornitore: Thermo Fisher Scientific

Descrizione: RevertAid™ H Minus First Strand cDNA Synthesis kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.