Hai cercato: Reagenti per Western Blotting

Fornitore: Thermo Fisher Scientific

Descrizione: SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

MSDS Certificati

Fornitore: Thermo Fisher Scientific

Descrizione: Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Fornitore: Thermo Fisher Scientific

Descrizione: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Codice catalogo: (PIER34035)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34035

Codice articolo locale: PIER34035

Descrizione: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

UOM: 1 * 1 g

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Fornitore: Thermo Fisher Scientific

Descrizione: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

MSDS

Fornitore: Thermo Fisher Scientific

Descrizione: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Codice catalogo: (PIER37574)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 37574

Codice articolo locale: PIER37574

Descrizione: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

UOM: 1 * 250 mL

Promo

Codice catalogo: (PIER34012)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34012

Codice articolo locale: PIER34012

Descrizione: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

UOM: 1 * 250 mL

Promo

Fornitore: AAT BIOQUEST

Descrizione: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

New Product

Fornitore: Thermo Fisher Scientific

Descrizione: Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Fornitore: Thermo Fisher Scientific

Descrizione: SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.

Fornitore: Thermo Fisher Scientific

Descrizione: The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

Codice catalogo: (PIER31490)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 31490

Codice articolo locale: PIER31490

Descrizione: Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

UOM: 1 * 10 mg

Promo

Codice catalogo: (PIER17075)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 17075

Codice articolo locale: PIER17075

Descrizione: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.

UOM: 1 * 1 KIT

Promo

Codice catalogo: (PIER34002)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: 34002

Codice articolo locale: PIER34002

Descrizione: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.

UOM: 1 * 1 KIT

Promo