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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signalling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signalling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-AGLS200550

Random mutagenesis kits geneMorph II

Descrizione: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
Codice catalogo: AGLS200550
UOM: 1 * 1 kt
Codice articolo fornitore: 200550
Codice articolo locale: AGLS200550
Fornitore: AGILENT
Product Image-AGLS200513

Mutagenesis kits QuikChange

Descrizione: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.
Codice catalogo: AGLS200513
UOM: 1 * 1 pezzi
Codice articolo fornitore: 200513
Codice articolo locale: AGLS200513
Fornitore: AGILENT
Product Image-K1231

PCR cloning kit, CloneJET

Descrizione: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.
Codice catalogo: K1231
UOM: 1 * 1 KIT
Codice articolo fornitore: K1231
Codice articolo locale: FERMK1231
Fornitore: Thermo Fisher Scientific
Image Unavailable-AGLS600191

Pfu DNA ligase

Descrizione: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
Codice catalogo: AGLS600191
UOM: 1 * 1 pezzi
Codice articolo fornitore: 600191
Codice articolo locale: AGLS600191
Fornitore: AGILENT
Image Unavailable-AGLS240205

PCR cloning kits, StrataClone

Descrizione: The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
Codice catalogo: AGLS240205
UOM: 1 * 1 kt
Codice articolo fornitore: 240205
Codice articolo locale: AGLS240205
Fornitore: AGILENT
Image Unavailable-AGLSG5900B

QuikChange HT protein engineering system

Descrizione: Protein directed evolution in just one step.
Codice catalogo: AGLSG5900B
UOM: 1 * 1 kt
Codice articolo fornitore: G5900B
Codice articolo locale: AGLSG5900B
Fornitore: AGILENT
Image Unavailable-AGLS200207

Packaging extracts, Gigapack III

Descrizione: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.
Codice catalogo: AGLS200207
UOM: 1 * 1 pezzi
Codice articolo fornitore: 200207
Codice articolo locale: AGLS200207
Fornitore: AGILENT
Product Image-EL0016

T4 DNA ligase

Descrizione: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Codice catalogo: EL0016
UOM: 1 * 1 KIT
Codice articolo fornitore: EL0016
Codice articolo locale: FERMEL0016
Fornitore: Thermo Fisher Scientific

Certificati


Product Image-GA1200-50MM

Gibson Assembly® Ultra, Master mixes

Descrizione: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.
Codice catalogo: GA1200-50MM
UOM: 1 * 50 Tests
Codice articolo fornitore: GA1200-50MM
Codice articolo locale: SYGEGA1200-50MM
Fornitore: SGI - DNA Synthetic Genomics

MSDS


Product Image-GA1100-10

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Descrizione: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.
Codice catalogo: GA1100-10
UOM: 1 * 10 Tests
Codice articolo fornitore: GA1100-10
Codice articolo locale: SYGEGA1100-10
Fornitore: SGI - DNA Synthetic Genomics

MSDS


Product Image-SYGEGA1100-4X50

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Descrizione: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.
Codice catalogo: SYGEGA1100-4X50
UOM: 1 * 50 Tests
Codice articolo fornitore: GA1100-4X50
Codice articolo locale: SYGEGA1100-4X50
Fornitore: SGI - DNA Synthetic Genomics
Product Image-K1271

LIC cloning and expression kits, aLICator™, Fermentas

Descrizione: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.
Codice catalogo: K1271
UOM: 1 * 1 SET
Codice articolo fornitore: K1271
Codice articolo locale: FERMK1271
Fornitore: Thermo Fisher Scientific
Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Descrizione: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.
Codice catalogo: GA1100-10MM
UOM: 1 * 10 Tests
Codice articolo fornitore: GA1100-10MM
Codice articolo locale: SYGEGA1100-10MM
Fornitore: SGI - DNA Synthetic Genomics

MSDS


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Ligation premix, Clonables™

Descrizione: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.
Codice catalogo: 70573-4
UOM: 1 * 2,5 mL
Codice articolo fornitore: 70573-4
Codice articolo locale: NOVG70573-4
Fornitore: Merck Millipore (Novagen)
Product Image-GA1200-10

Gibson Assembly® Ultra kit

Descrizione: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.
Codice catalogo: GA1200-10
UOM: 1 * 10 Tests
Codice articolo fornitore: GA1200-10
Codice articolo locale: SYGEGA1200-10
Fornitore: SGI - DNA Synthetic Genomics

MSDS


Image Unavailable-70010-3

T7Select® phage display system, Novagen®

Descrizione: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).
Codice catalogo: 70010-3
UOM: 1 * 1 KIT
Codice articolo fornitore: 70010-3
Codice articolo locale: NOVG70010-3
Fornitore: Merck Millipore (Novagen)
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