Hai cercato: L-Cisteina

Fornitore: Quantabio

Descrizione: La One-Step Kit RT-PCR qScript ™ XLT è un sistema conveniente e altamente sensibile per l'amplificazione di RNA modelli fino a 2 kb. sintesi del cDNA e l'amplificazione PCR vengono effettuate nello stesso tubo senza aprire tra procedure. Questo sistema è stato ottimizzato per garantire la massima RT-PCR efficienza, sensibilità e specificità.

Certificati

Fornitore: AGILENT

Descrizione: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Fornitore: Quantabio

Descrizione: I kit qScript™ cDNA Synthesis contengono qScript™ Reverse Transcriptase, una miscela di MMLV ingegnerizzata e proteina per l'inibizione della ribonucleasi, ottimizzata per una sintesi di cDNA sensibile e affidabile. I reagenti per la sintesi di cDNA sono forniti in diversi formati supermix e in kit per soddisfare le esigenze specifiche delle singole applicazioni.

MSDS

Fornitore: Quantabio

Descrizione: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

MSDS

Fornitore: Cytiva

Descrizione: Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

Fornitore: Thermo Fisher Scientific

Descrizione: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Codice catalogo: (71978-3)

Fornitore: Merck Millipore (Novagen)

Codice articolo fornitore: 71978-3

Codice articolo locale: NOVG71978-3

Descrizione: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

Promo

Fornitore: Quantabio

Descrizione: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Fornitore: Thermo Fisher Scientific

Descrizione: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Fornitore: Thermo Fisher Scientific

Descrizione: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Certificati

Fornitore: Thermo Fisher Scientific

Descrizione: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Codice catalogo: (27-9261-01)

Fornitore: Cytiva

Codice articolo fornitore: 27-9261-01

Codice articolo locale: GEHE27-9261-01

Descrizione: Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for <i>in vitro</i> amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

UOM: 1 * 55 Tests

Promo

Codice catalogo: (K1612)

Fornitore: Thermo Fisher Scientific

Codice articolo fornitore: K1612

Codice articolo locale: FERMK1612

Descrizione: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

Promo

Fornitore: Thermo Fisher Scientific

Descrizione: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Fornitore: Thermo Fisher Scientific

Descrizione: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

MSDS

Codice catalogo: (27-9264-01)

Fornitore: Cytiva

Codice articolo fornitore: 27-9264-01

Codice articolo locale: GEHE27-9264-01

Descrizione: Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

UOM: 1 * 50 Tests

MSDS Promo